Bearpaw Phylly rznJpviu7X

SKU23326493
Bearpaw Phylly rznJpviu7X
Bearpaw Phylly

The National Endowment for the Arts is an independent federal agency that funds, promotes, and strengthens the creative capacity of our communities by providing all Americans with diverse opportunities for arts participation.

View larger version:
FIG 3

Effects of SLFN5 knockdown on RCC morphology. (A) 786-0 cells were transiently transfected with control siRNA or SLFN5 siRNA, and after cell lysis, total lysates were resolved by SDS-PAGE and immunoblotted with an anti-SLFN5 antibody or an anti-GAPDH antibody, as indicated. (B) 786-0 cells stably expressing control shRNA-GFP or SLFN5 shRNA-GFP were lysed, and total cell lysates were resolved by SDS-PAGE and immunoblotted with anti-SLFN5 or anti-GAPDH antibodies, as indicated. (C) 786-0 cells were transiently transfected with control (CTRL) siRNA or SLFN5 siRNA. Forty-eight hours later, cells were imaged by phase-contrast microscopy at low (left panels) and high (right panels) magnifications. (D) 786-0 cells seeded onto glass coverslips were transiently transfected with control siRNA or SLFN5 siRNA with Lipofectamine RNAiMAX; at 48 h after transfection, the cells were fixed, permeabilized, and stained. Confocal fluorescence microscopy of focal adhesion and actin cytoskeleton was performed with triple labeling, using tetramethyl rhodamine-isothiocyanate (TRITC)-conjugated phalloidin (lower right panels), antivinculin monoclonal antibody (lower left panels), and 4′,6-diamidino-2-phenylindole (DAPI) (upper right panels). Overlay of fluorescent signals is shown in upper left panels. Images are representative areas of the entire coverslip analyzed. Bars, 50 μm. (E) 786-0 cells stably expressing control shRNA-GFP or SLFN5 shRNA-GFP were seeded for 24 h onto glass coverslips. Cells were then fixed, permeabilized, and stained with Alexa Fluor 568-labeled phalloidin to detect cytoskeletal F-actin by confocal fluorescence microscopy. Representative areas showing the F-actin structure are shown. (F) Diameter distributions of 786-0 control shRNA-GFP and SLFN5 shRNA-GFP cells were measured using the Scepter 2.0 cell counter. Differences in cell diameter are plotted on a bar graph and represent means ± standard errors from three independent counts.

View larger version:
FIG 4

Knockdown of , , or does not result in loss of stress fibers and reduction in cell size. (A to E) 786-0 cells seeded for 24 h onto glass coverslips were transiently transfected with control siRNA (A), SLFN5 siRNA (B), SLFN11 siRNA (C), SLFN12 siRNA (D), or SLFN13 siRNA (E) using Lipofectamine RNAiMAX; at 48 h after transfection, the cells were fixed, permeabilized and incubated with 4′,6-diamidino-2-phenylindole and Alexa Fluor 568-labeled phalloidin. Overlays of representative fluorescence signals are shown. (F) 786-0 cells were transiently transfected, respectively, with SLFN5, SLFN11, SLFN12, or SLFN13 siRNA or control siRNA, and mRNA expression for different SLFNs was measured by real-time RT-PCR, using a GAPDH primer as a control. Data are expressed as the fold change over controls and represent means ± standard errors of results from three independent experiments.

View larger version:
FIG 5

SLFN5 knockdown does not affect cell proliferation. (A) Equal numbers of 786-0 control siRNA- and SLFN5 siRNA-transfected cells were cultured for the indicated times. After 1, 2, 4, 5, and 6 days of incubation, cell proliferation was assessed by WST assays. Data are expressed as means ± standard errors of results from three independent experiments. (B) Control shRNA-GFP and SLFN5 shRNA-GFP 786-0 cells were seeded in culture dishes and harvested after 3 or 4 days. Cell counts were obtained using the trypan blue dye exclusion test. Data are expressed as means ± standard errors of results from three independent experiments.

View larger version:
FIG 6

Effect of SLFN5 overexpression on cell morphology. (A) 786-0 pLVX/tetONE-puro-SLFN5-Myc-Flag tag-transduced cells were treated with doxycycline (Dox) as indicated, and after cell lysis, proteins were resolved by SDS-PAGE and immunoblotted with an anti-SLFN5 antibody and an antitubulin antibody. (B) The same cells as used in panel A were treated with doxycycline for 72 h and then seeded onto 0.2% gelatin-coated glass coverslips. Cells were then fixed, permeabilized, and stained with Alexa Fluor 568-labeled phalloidin and 4′,6-diamidino-2-phenylindole (DAPI) to detect cytoskeletal F-actin and nuclei by confocal fluorescence microscopy. Representative areas showing the F-actin structure and nuclei are shown. Bars, 100 μm. UT, untreated.

Since these findings suggest that SLFN5 controls pathways involved in cytoskeletal rearrangement, we performed RNA-seq analysis to examine the global effects of SLFN5 knockdown on cellular pathways and to identify putative effector elements. RNA-seq analysis showed that 956 genes were upregulated and 570 genes were downregulated following SLFN5 knockdown ( Alice Olivia Sasha sKzRkyE
). We determined that cancer-related pathways were the highest-ranked pathways affected by SLFN5 knockdown ( Anne Klein Harmonie CW8Sg3
). Additionally, pathways involved in focal adhesion and cell junctions were also regulated by SLFN5 ( Fig. 7B ). Overall, elements of pathways involved in the regulation of cell morphology were found to be the most affected in another independent pathway analysis ( Table 1 ). Significantly, the matrix metalloproteinase 1 gene ( MMP-1 ) and MMP-13 were among the top dysregulated genes by SLFN5 knockdown in the RNA-seq data ( Fig. 7C ).

Accessibility links

Nine West Mary cvEOf
Search

Menu

Sections
US Canada
US Canada
By Tara McKelvey White House reporter, BBC News
5 February 2016

Share this with

Facebook

Messenger

Twitter

Pinterest

WhatsApp

LinkedIn

Copy this link

Chinese Laundry Krush Boot n8BmyaJ0

These are external links and will open in a new window

Related Topics
Why has Trump caught Clinton in the polls?
24 May 2016
The Democrats' election nightmare
26 May 2016
Clinton emails - what's it all about?
6 November 2016
Video Hillary Clinton: Her video timeline
12 April 2015

Two Democratic candidates for the US presidency, Senator Bernie Sanders and Hillary Clinton, are fighting over the term "progressive". But what does the word really mean?

In a CNN town hall on 3 February in New Hampshire Mr Sanders and Mrs. Clinton argued over what the word "progressive" means and who has the right to describe themselves in this way.

Mr Sanders said she's not a liberal when it comes to foreign policy and other issues. She disagreed with him, saying that she's "a progressive who likes to get things done". She added that she was "amused" that he'd "set himself up as the gatekeeper of who gets to be a progressive".

So what is a progressive?

Politicians, activists and others disagree about what the word means. Historians concede that there's no precise definition. Still they say that in general a progressive fits certain criteria.

A progressive is someone who wants to see more economic and social equality - and hopes to see more gains in feminism and gay rights. They're also supportive of social programmes directed by the state - and they'd like social movements have more power in the US.

Within the realm of progressive, however, there are different, warring factions, explains David Greenberg, the author of a book called Republic of Spin: An Inside History of the American Presidency.

One group is dominated by activists from social movements such as Occupy Wall Street and Black Lives Matter, he says, and the other is led by those who belong to the left wing of the Democratic Party (and aren't part of a social movement or cause).

Pretty much all of of these progressives "view politics as a bottom-up progress", says Julian Zelizer, an historian at Princeton, and they support the fight for social change. (Though not everybody is on the streets, clamouring for it.)

Get Started

Thank you for reactivating your COLLEGEdata account!

Get Started
© 2018 1st Financial Bank USA
For Students Age 18 and Older

Have you received a Personal Invitation to apply for a Student Credit card?

Click here

Learn how to qualify for a Personal Invitation to apply for a Student Credit Card

Click here

1st Financial Bank believes students who pick colleges wisely will also want to learn how to use credit cards wisely.

4

2700

3

/cs/admissions/admissions_profile.jhtml
/cs/admissions/admissions_tracker_result.jhtml
2022
added for class of 2024 - 2 hours ago updated their profile for class of 2024 - 2 hours ago 2 Admissions Profiles were added to today applied to - 2 hours ago
College Match

Find the right colleges for you!

College Chances

Will you get in? Find out!

Admissions Tracker

See who got in where, who's applying how you compare!

Financial Aid Tracker

See who's getting financial aid, how much how you stack up.

Scholarship Finder

Find money for college!

EFC Calculator

Calculate your Expected Family Contribution.

College Net Price Calculator

See your true out-of-pocket cost to attend any college.

Compare Financial Aid Awards

Find out which college is offering you the best deal.

JUNE 2, 2018 SAT SAT Subject Tests test date
JUNE 9 ACT test date
JUNE 15 Registration deadline for July 14 ACT
JUNE 30 Last date to submit FAFSA for federal aid for 2017-18 school year
JULY 14, 2018 ACT test date (not in NY)
AUGUST 25, 2018 SAT SAT Subject Tests test date
SEPTEMBER 8, 2018 ACT test date
SEPTEMBER 15 Last date to submit FAFSA corrections for federal aid for 2017-18 school year
OCTOBER 1, 2018 First date to submit FAFSA for 2019-20 school year
OCTOBER 6 SAT SAT Subject Tests test date
OCTOBER 27 ACT test date
NOVEMBER 3, 2018 SAT SAT Subject Tests test date
DECEMBER 1, 2018 SAT SAT Subject Tests test date
DECEMBER 8 ACT test date
FEBRUARY 9, 2019 ACT test date (not in NY)
MARCH 9, 2019 SAT test date
APRIL 13, 2019 ACT test date
MAY 4, 2019 SAT SAT Subject Tests test date
JUNE 1, 2019 SAT SAT Subject Tests test date
JUNE 8 ACT test date
JUNE 30 Last date to submit FAFSA for federal aid for 2018-19 school year
JULY 13, 2019 ACT test date (not in NY)

Log in to see all of your saved colleges, scholarships, articles, profiles and searches in one place. Access your Dashboard from any page.

New User?
for your free account.
COLLEGEdata Dollars (CD$) are points you earn by completing certain COLLEGEdata activities.
Sign up to earn 2000 CD$! Once you earn 2500 CD$ you can redeem them for U.S. Dollars.
For more information, click .

Ways to earn COLLEGEdata Dollars:

COLLEGEdata Dollars (CD$) are points you earn by completing certain COLLEGEdata activities. The maximum number of CD$ you can redeem is 5000. Once you have earned at least 2500 CD$, you can redeem them for $25, which will be provided to you on a Loyalty Card, and once you earn another 2500 CD$, you can redeem those CD$ for a second $25, which will also be provided to you on a Loyalty Card.

Complete certain COLLEGEdata activities (for example, signing up, starting your Admissions Profile, searching for colleges, calculating your chances for admission, searching for scholarships, updating your Profile with your admission decisions). Each activity is worth a specific amount of points (CD$). You can redeem the points you earn for U.S. Dollars that will be issued to you in the form of a 1st Financial Bank USA Loyalty Mastercard®.

You can earn CD$ by completing certain COLLEGEdata activities. As soon as you sign up and activate your COLLEGEdata account, explore COLLEGEdata and begin completing COLLEGEdata activities to earn points.

Here is a full list of COLLEGEdata activities for which you may earn CD$ and the number of CD$ you can earn by completing each activity.*

Deep Root's database on the open Amazon S3 storage server highlights the years-long effort by companies to stockpile data about American voters. The database includes information from the 2008, 2012 and 2016 presidential campaigns. The 2016 files were not as comprehensive as preceding years.

The leaked data included posts scraped from Reddit. The text from those posts could be used for training computers to recognize language sentiment or to watch specific subreddits to see how people were feeling about political topics.

Some of the exposed information, like voter registration, is public record, but states have different ways of letting people access it and rules on how it can be used.

Related: 4 myths -- and facts -- about online security

This is the third time Vickery has found a huge portion of the national voter registration database leaked online. Amazon buckets -- where data is stored -- are private by default. Someone would have to configure the bucket to be public in order to be exposed.

What's also unknown is whether Vickery was the first person to notice the exposed information.

Laycee Laser Perforated Block Heel Sandals M8RjF

If a bad guy accessed that data, it could be used for stealing someone's identity, stalking individuals or used as leverage for social engineering purposes (that is, tricking something like a phone company into giving someone else your data).

"There's a lot of people hunting for publicly exposed buckets for nefarious purposes," he said.

CNNMoney (San Francisco) First published June 19, 2017: 8:54 PM ET
Paid Content
Paid Content
More from CNN Money
Earth Angel 47TgdCx
Paid Content
Ivanka Trump Ketty wBkE56yWhd
Facebook apologizes for bug that temporarily unblocked people
(this site)
Tesla finally hits Elon Musk's target for the Model 3
(this site)
Barnes Noble fires CEO for violating company policy
(this site)
New York Times DC reporter who dated Senate intelligence staffer…
(this site)
Remembering the Capital Gazette victims
(News)
Facebook apologizes for bug that temporarily unblocked people
(this site)
Tesla finally hits Elon Musk's target for the Model 3
(this site)
Barnes Noble fires CEO for violating company policy
(this site)
New York Times DC reporter who dated Senate intelligence staffer…
(this site)
Remembering the Capital Gazette victims
(News)
by Outbrain
How Netflix is everything to everyone
(Else - Videos)
The left is raging. Is the media listening?
(News)
Facts you might not know about July 4th
(News)
The truth about the retail apocalypse
(News)
How Netflix is everything to everyone
(Else - Videos)
The left is raging. Is the media listening?
(News)
Facts you might not know about July 4th
(News)
The truth about the retail apocalypse
(News)

CNNMoney Sponsors

Paid Partner
Paid Partner

follow us!

Most stock quote data provided by BATS. Market indices are shown in real time, except for the DJIA, which is delayed by two minutes. All times are ET. Miz Mooz Storm 79TTfj2UKr
. Morningstar: © 2018 Morningstar, Inc. All Rights Reserved. Factset: FactSet Research Systems Inc. 2018 . All rights reserved. Chicago Mercantile Association: Certain market data is the property of Chicago Mercantile Exchange Inc. and its licensors. All rights reserved. Dow Jones: The Dow Jones branded indices are proprietary to and are calculated, distributed and marketed by DJI Opco, a subsidiary of SP Dow Jones Indices LLC and have been licensed for use to SP Opco, LLC and CNN. Standard Poor's and SP are registered trademarks of Standard Poor's Financial Services LLC and Dow Jones is a registered trademark of Dow Jones Trademark Holdings LLC. All content of the Dow Jones branded indices © SP Dow Jones Indices LLC 2018 and/or its affiliates.

Contact us
Advertisers
About us
Quick links
Mobile and tools
Partners

National advertising powered by Mediative.com . Yellow Pages TM , Walking Fingers Design TM , YP.ca TM , Find. Design TM , YellowPages.ca TM , Canada411 TM and YP Shopwise TM are trademarks of Yellow Pages Digital Media Solutions Limited in Canada. All other trademarks are the property of their respective owners. Copyright © 2018 Yellow Pages Digital Media Solutions Limited . All Rights Reserved. 5.25.0.0 (rev 20180627.1124)